photo by:TPittawayM. Vila, N. Mari Mena, S-H. Yen & C. Lopez-Vaamonde (2009)
Conservation Genetics. DOI 10.1007/s10592-009-9905-1
本研究發展出十個西班牙月蛾的多型性微衛星體基因座。作者對來自一個西班牙族群(Els-Ports-de-Beseit, Catalonia)的20個個體、39個來自法國山區的個體和6個來自西班牙其他地點的個體進行多型性的評估。整體而言，基因座上的對偶基因數量範圍為 5-24個。 Els-Ports-de-Beseit族群每個基因座上的平均對偶基因數量是 9.80 (SD = 4.32)，異型合子比例為 0.71 (SD = 0.226)，異型合子比例的期望值是0.788 (SD = 0.146)。Catalonia族群的基因型頻率符合哈溫平衡，且沒有觀察到連鎖不平衡的證據。由這組標誌所得之多基因座基因型將可應用在判斷此高度保育蛾種的遺傳岐異度與族群間的分化。有一些基因座在兩種近緣種上被找到且具有多型性: 在Actias neidhoeferi (姬長尾水青蛾)上有兩個基因座，A. luna則有三個。
Abstract Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals from bone population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from 5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was 0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna.
Graellsia isabelae 研究群: Institut National de la Recherche Agronomique (INRA) - Centre d'Orléans 網站
Abstract Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals from bone population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from 5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was 0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna.
Graellsia isabelae 研究群: Institut National de la Recherche Agronomique (INRA) - Centre d'Orléans 網站
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